A.I.S.E. research published on performance of In Vitro method for eye effects

A.I.S.E. has previously sponsored research to investigate the usefulness of in vitro test methods for eye irritation/eye damage, to ensure reliable and meaningful classification and labelling for detergent and cleaning product formulations without animal testing on finished products.  The importance of correct classification was highlighted in a paper from A.I.S.E. in 2019, which compared eye hazard classifications with effects following accidental exposure to detergent and maintenance products and found that classification was a poor predictor of the effects in practice.

In new research published in the November 2021 volume of Regulatory Toxicology and Pharmacology, A.I.S.E. has investigated the inter-laboratory performance of Isolated Chicken Eye (ICE) histopathology scoring as a method to predict GHS/EU CLP Cat.1 classification for surfactants and non-extreme pH detergents.  

The research found that ICE histopathology:
  • is suitable to predict Cat. 1 effects of surfactants and non-extreme pH detergents.
  • increases accuracy vs. ICE alone to predict Cat. 1 effects of surfactants and non-extreme pH detergents.
  • is reproducible between pathologists of independent laboratories to predict serious eye damage.
  • is reproducible over time to predict serious eye damage of surfactants and non-extreme pH detergents.

The paper also makes recommendations for test facilities to ensure the reproducible use of ICE histopathology.

The article is available at: https://doi.org/10.1016/j.yrtph.2021.105044